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polyclonal rabbit anti human fibrinogen  (Thermo Fisher)


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    Structured Review

    Thermo Fisher polyclonal rabbit anti human fibrinogen
    Polyclonal Rabbit Anti Human Fibrinogen, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyclonal+rabbit+anti+human+fibrinogen/Fibrinogen/pm35291561-289-40-54
    Average 99 stars, based on 1 article reviews
    polyclonal rabbit anti human fibrinogen - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Bicinchoninic Acid Protein Assay:

    Article Title: Airway Obstruction Due to Bronchial Vascular Injury after Sulfur Mustard Analog Inhalation
    Article Snippet: IgM concentrations were quantitatively measured in the BALF according to a standard ELISA protocol (Bethyl Laboratories, Inc., Montgomery, TX). .. Total protein was measured in BALF, using the bicinchoninic acid (BCA) protein assay (Pierce, Rockville, IL). β-Fibrin Detection by Western Blot β-Fibrin was detected in BALF by Western blot with polyclonal rabbit anti-human fibrinogen (DAKO, Glostrup, Denmark) or mouse monoclonal β-actin (Sigma), followed by incubation with horseradish peroxidase–conjugated goat anti-rabbit IgG (Bio-Rad, Hercules, CA) or horseradish peroxidase–conjugated rabbit anti-mouse IgG (Sigma), respectively. .. Rat fibrinogen (Sigma-Aldrich Chemical Co.) was used to generate fibrin standards.

    Article Title: Airway Obstruction Due to Bronchial Vascular Injury after Sulfur Mustard Analog Inhalation
    Article Snippet: IgM concentrations were quantitatively measured in the BALF according to a standard ELISA protocol (Bethyl Laboratories, Inc., Montgomery, TX). .. Total protein was measured in BALF, using the bicinchoninic acid (BCA) protein assay (Pierce, Rockville, IL). b-Fibrin Detection by Western Blot b-Fibrin was detected in BALF by Western blot with polyclonal rabbit anti-human fibrinogen (DAKO, Glostrup, Denmark) or mouse monoclonal b-actin (Sigma), followed by incubation with horseradish peroxidase–conjugated goat anti-rabbit IgG (Bio-Rad, Hercules, CA) or horseradish peroxidase–conjugated rabbit anti-mouse IgG (Sigma), respectively. .. Rat fibrinogen (Sigma-Aldrich Chemical Co.) was used to generate fibrin standards.

    Western Blot:

    Article Title: Airway Obstruction Due to Bronchial Vascular Injury after Sulfur Mustard Analog Inhalation
    Article Snippet: IgM concentrations were quantitatively measured in the BALF according to a standard ELISA protocol (Bethyl Laboratories, Inc., Montgomery, TX). .. Total protein was measured in BALF, using the bicinchoninic acid (BCA) protein assay (Pierce, Rockville, IL). β-Fibrin Detection by Western Blot β-Fibrin was detected in BALF by Western blot with polyclonal rabbit anti-human fibrinogen (DAKO, Glostrup, Denmark) or mouse monoclonal β-actin (Sigma), followed by incubation with horseradish peroxidase–conjugated goat anti-rabbit IgG (Bio-Rad, Hercules, CA) or horseradish peroxidase–conjugated rabbit anti-mouse IgG (Sigma), respectively. .. Rat fibrinogen (Sigma-Aldrich Chemical Co.) was used to generate fibrin standards.

    Article Title: Airway Obstruction Due to Bronchial Vascular Injury after Sulfur Mustard Analog Inhalation
    Article Snippet: IgM concentrations were quantitatively measured in the BALF according to a standard ELISA protocol (Bethyl Laboratories, Inc., Montgomery, TX). .. Total protein was measured in BALF, using the bicinchoninic acid (BCA) protein assay (Pierce, Rockville, IL). b-Fibrin Detection by Western Blot b-Fibrin was detected in BALF by Western blot with polyclonal rabbit anti-human fibrinogen (DAKO, Glostrup, Denmark) or mouse monoclonal b-actin (Sigma), followed by incubation with horseradish peroxidase–conjugated goat anti-rabbit IgG (Bio-Rad, Hercules, CA) or horseradish peroxidase–conjugated rabbit anti-mouse IgG (Sigma), respectively. .. Rat fibrinogen (Sigma-Aldrich Chemical Co.) was used to generate fibrin standards.

    Incubation:

    Article Title: Airway Obstruction Due to Bronchial Vascular Injury after Sulfur Mustard Analog Inhalation
    Article Snippet: IgM concentrations were quantitatively measured in the BALF according to a standard ELISA protocol (Bethyl Laboratories, Inc., Montgomery, TX). .. Total protein was measured in BALF, using the bicinchoninic acid (BCA) protein assay (Pierce, Rockville, IL). β-Fibrin Detection by Western Blot β-Fibrin was detected in BALF by Western blot with polyclonal rabbit anti-human fibrinogen (DAKO, Glostrup, Denmark) or mouse monoclonal β-actin (Sigma), followed by incubation with horseradish peroxidase–conjugated goat anti-rabbit IgG (Bio-Rad, Hercules, CA) or horseradish peroxidase–conjugated rabbit anti-mouse IgG (Sigma), respectively. .. Rat fibrinogen (Sigma-Aldrich Chemical Co.) was used to generate fibrin standards.

    Article Title: Airway Obstruction Due to Bronchial Vascular Injury after Sulfur Mustard Analog Inhalation
    Article Snippet: IgM concentrations were quantitatively measured in the BALF according to a standard ELISA protocol (Bethyl Laboratories, Inc., Montgomery, TX). .. Total protein was measured in BALF, using the bicinchoninic acid (BCA) protein assay (Pierce, Rockville, IL). b-Fibrin Detection by Western Blot b-Fibrin was detected in BALF by Western blot with polyclonal rabbit anti-human fibrinogen (DAKO, Glostrup, Denmark) or mouse monoclonal b-actin (Sigma), followed by incubation with horseradish peroxidase–conjugated goat anti-rabbit IgG (Bio-Rad, Hercules, CA) or horseradish peroxidase–conjugated rabbit anti-mouse IgG (Sigma), respectively. .. Rat fibrinogen (Sigma-Aldrich Chemical Co.) was used to generate fibrin standards.

    Marker:

    Article Title: APOE4 accelerates advanced-stage vascular and neurodegenerative disorder in old Alzheimer’s mice via cyclophilin A independently of amyloid-β
    Article Snippet: After incubation with primary antibodies, sections were washed in PBS and incubated with fluorophore-conjugated secondary antibodies (see below) and then mounted onto slides with fluorescence mounting medium (Dako). .. The following primary and secondary antibodies were used, respectively: for pericyte coverage, polyclonal goat anti-mouse aminopeptidase N/ANPEP (CD13) (R&D systems AF2335, 1:100 dilution) and Alexa Fluor 488- or 568-conjugated donkey anti-goat (Invitrogen, A-11055 or A-11057, 1:500 dilution); for fibrin/fibrinogen, polyclonal rabbit anti-human fibrinogen (Dako A0080, 1:500 dilution) and Alexa Fluor 488-conjugated donkey anti-rabbit (Invitrogen, A-21206,1:500 dilution); for tight junction proteins (zonula occludens), rabbit anti-mouse ZO-1 (Invitrogen 40–2200, 1:200 dilution) and Alexa Fluor 568-conjugated donkey anti-rabbit (Invitrogen A-10042, 1:500 dilution); and for occludin, mouse anti-mouse occludin (Invitrogen clone OC-3F10 no. 33–1500, 1:100 dilution) and Alexa Fluor 488-conjugated donkey anti-mouse (Invitrogen A-21202, 1:500 dilution); for CypA, rabbit anti-mouse CypA (Abcam ab42408, 1:100 dilution) and Alexa Fluor 488 or 568-conjugated donkey anti-rabbit (Invitrogen A-21206 or A-10042, 1:500 dilution); for MMP9, rabbit anti-mouse MMP9 (Abcam, ab38898, 1:100 dilution) and Alexa Fluor 488 or 568-conjugated donkey anti-rabbit (Invitrogen A-21206 or A-10042, 1:500 dilution); for amyloid-β, rabbit anti-human β-amyloid (Cell Signaling 8243S, 1:500 dilution) and Alexa Fluor 647-conjugated donkey anti-rabbit (Invitrogen A-31573,1:500 dilution); for neurofilament, mouse monoclonal anti-axonal neurofilament marker (SMI312) (BioLegend SMI312, 1:500 dilution) and Alexa Fluor 488-conjugated donkey anti-mouse (Invitrogen A-21202, 1:500 dilution); and for neuronal, polyclonal rabbit anti-NeuN (Millipore ABN78, 1:500 dilution) and Alexa Fluor 488 or 568-conjugated donkey anti-rabbit (Invitrogen, A-21206 or A-10042; 1:500 dilution). .. To visualize brain microvessels, sections were incubated with Dylight 488, 594 or 647-conjugated Lycopersicon esculentum lectin (Vector Labs, DL-1174; 1:200 dilution) for 1 h. Sections were imaged with a Zeiss LSM 510 confocal laser-scanning microscope or BZ9000 fluorescence microscope.

    Article Title: APOE4 accelerates advanced-stage vascular and neurodegenerative disorder in old Alzheimer's mice via cyclophilin A independently of amyloid-β.
    Article Snippet: After incubation with primary antibodies, sections were washed in PBS and incubated with fluorophore-conjugated secondary antibodies (see below) and then mounted onto slides with fluorescence mounting medium (Dako). .. The following primary and secondary antibodies were used, respectively: for pericyte coverage, polyclonal goat anti-mouse aminopeptidase N/ANPEP (CD13) (R&D systems AF2335, 1:100 dilution) and Alexa Fluor 488- or 568-conjugated donkey anti-goat (Invitrogen, A-11055 or A-11057, 1:500 dilution); for fibrin/ fibrinogen, polyclonal rabbit anti-human fibrinogen (Dako A0080, 1:500 dilution) and Alexa Fluor 488-conjugated donkey anti-rabbit (Invitrogen, A-21206,1:500 dilution); for tight junction proteins (zonula occludens), rabbit anti-mouse ZO-1 (Invitrogen 40–2200, 1:200 dilution) and Alexa Fluor 568-conjugated donkey antirabbit (Invitrogen A-10042, 1:500 dilution); and for occludin, mouse anti-mouse occludin (Invitrogen clone OC-3F10 no. 33-1500, 1:100 dilution) and Alexa Fluor 488-conjugated donkey anti-mouse (Invitrogen A-21202, 1:500 dilution); for CypA, rabbit anti-mouse CypA (Abcam ab42408, 1:100 dilution) and Alexa Fluor 488 or 568-conjugated donkey anti-rabbit (Invitrogen A-21206 or A-10042, 1:500 dilution); for MMP9, rabbit anti-mouse MMP9 (Abcam, ab38898, 1:100 dilution) and Alexa Fluor 488 or 568-conjugated donkey anti-rabbit (Invitrogen A-21206 or A-10042, 1:500 dilution); for amyloid-β, rabbit anti-human β-amyloid (Cell Signaling 8243S, 1:500 dilution) and Alexa Fluor 647-conjugated donkey antirabbit (Invitrogen A-31573,1:500 dilution); for neurofilament, mouse monoclonal anti-axonal neurofilament marker (SMI312) (BioLegend SMI312, 1:500 dilution) and Alexa Fluor 488-conjugated donkey anti-mouse (Invitrogen A-21202, 1:500 dilution); and for neuronal, polyclonal rabbit anti-NeuN (Millipore ABN78, 1:500 dilution) and Alexa Fluor 488 or 568-conjugated donkey anti-rabbit (Invitrogen, A-21206 or A-10042; 1:500 dilution). .. To visualize brain microvessels, sections were incubated with Dylight 488, 594 or 647-conjugated Lycopersicon esculentum lectin (Vector Labs, DL-1174; 1:200 dilution) for 1 h. Sections were imaged with a Zeiss LSM 510 confocal laser-scanning microscope or BZ9000 fluorescence microscope.

    other:

    Article Title: APOE4 accelerates advanced-stage vascular and neurodegenerative disorder in old Alzheimer's mice via cyclophilin A independently of amyloid-β.
    Article Snippet: To visualize brain microvessels, sections were incubated with Dylight 488, 594 or 647-conjugated Lycopersicon esculentum lectin (Vector Labs, DL-1174; 1:200 dilution) for 1 h. Sections were imaged with a Zeiss LSM 510 confocal laser-scanning microscope or BZ9000 fluorescence microscope.



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    Results of western blot analysis of patient’s plasma proteins after snakebite, over time. Plasma proteins were separated by SDS-PAGE under reduced conditions followed by western blot analysis using <t>polyclonal</t> anti-human fibrinogen antibodies (DAKO, HPST, Prague, Czech Republic). Western blot analysis of human fibrinogen in patients’ plasma after bites by Atheris nitschei ( a ), Calloselasma rhodostoma ( b ), Crotalus adamenteus case 1 ( c ). MW—protein molecular weight marker (Thermo Scientific TM , Spectra TM Multicolor Broad Range Protein Ladder, Life Technologies Czech Republic Ltd., Prague, Czech Republic). Molecular masses (kDa) are indicated on the left side. FBG—human fibrinogen standard. The sample number is provided in the top line and hours since bite in the bottom line. Red arrows mark fibrinogen fragments induced by snakebite. The chains of human fibrinogen standards are highlighted with red square boxes. Note: Samples 1 and 2 in ( a ) are missing. They were not analyzed by Western blot.
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    Image Search Results


    Results of western blot analysis of patient’s plasma proteins after snakebite, over time. Plasma proteins were separated by SDS-PAGE under reduced conditions followed by western blot analysis using polyclonal anti-human fibrinogen antibodies (DAKO, HPST, Prague, Czech Republic). Western blot analysis of human fibrinogen in patients’ plasma after bites by Atheris nitschei ( a ), Calloselasma rhodostoma ( b ), Crotalus adamenteus case 1 ( c ). MW—protein molecular weight marker (Thermo Scientific TM , Spectra TM Multicolor Broad Range Protein Ladder, Life Technologies Czech Republic Ltd., Prague, Czech Republic). Molecular masses (kDa) are indicated on the left side. FBG—human fibrinogen standard. The sample number is provided in the top line and hours since bite in the bottom line. Red arrows mark fibrinogen fragments induced by snakebite. The chains of human fibrinogen standards are highlighted with red square boxes. Note: Samples 1 and 2 in ( a ) are missing. They were not analyzed by Western blot.

    Journal: Toxins

    Article Title: Fibrinogenolysis in Venom-Induced Consumption Coagulopathy after Viperidae Snakebites: A Pilot Study

    doi: 10.3390/toxins14080538

    Figure Lengend Snippet: Results of western blot analysis of patient’s plasma proteins after snakebite, over time. Plasma proteins were separated by SDS-PAGE under reduced conditions followed by western blot analysis using polyclonal anti-human fibrinogen antibodies (DAKO, HPST, Prague, Czech Republic). Western blot analysis of human fibrinogen in patients’ plasma after bites by Atheris nitschei ( a ), Calloselasma rhodostoma ( b ), Crotalus adamenteus case 1 ( c ). MW—protein molecular weight marker (Thermo Scientific TM , Spectra TM Multicolor Broad Range Protein Ladder, Life Technologies Czech Republic Ltd., Prague, Czech Republic). Molecular masses (kDa) are indicated on the left side. FBG—human fibrinogen standard. The sample number is provided in the top line and hours since bite in the bottom line. Red arrows mark fibrinogen fragments induced by snakebite. The chains of human fibrinogen standards are highlighted with red square boxes. Note: Samples 1 and 2 in ( a ) are missing. They were not analyzed by Western blot.

    Article Snippet: The FBG immunodetection was performed using rabbit anti-human fibrinogen polyclonal antibody (DAKO, HPST, Prague, Czech Republic; dilution 1:50,000 in 0.1% Tween/Tris-buffered saline) for 1 h at room temperature.

    Techniques: Western Blot, SDS Page, Molecular Weight, Marker

    Results of western blot analysis of patients’ plasma proteins after snakebite over time. Plasma proteins were separated by SDS-PAGE under reduced conditions followed by western blot analysis using polyclonal anti-human fibrinogen antibodies (DAKO, HPST, Prague, Czech Republic). Western blot analysis of human fibrinogen in patients’ plasma after bites by Crotalus adamanteus case 2 ( a ), Echis coloratus ( b ), Macrovipera schweizeri ( c ). MW—protein molecular weight marker (Thermo Scientific TM , Spectra TM Multicolor Broad Range Protein Ladder, Life Technologies Czech Republic Ltd., Prague, Czech Republic). Molecular masses (kDa) are indicated on the left side. FBG—human fibrinogen standard. The sample number is provided in the top line and hours since bite in the bottom line. Red arrows mark fibrinogen fragments induced by snakebite. The chains of human fibrinogen standards are highlighted with red square boxes.

    Journal: Toxins

    Article Title: Fibrinogenolysis in Venom-Induced Consumption Coagulopathy after Viperidae Snakebites: A Pilot Study

    doi: 10.3390/toxins14080538

    Figure Lengend Snippet: Results of western blot analysis of patients’ plasma proteins after snakebite over time. Plasma proteins were separated by SDS-PAGE under reduced conditions followed by western blot analysis using polyclonal anti-human fibrinogen antibodies (DAKO, HPST, Prague, Czech Republic). Western blot analysis of human fibrinogen in patients’ plasma after bites by Crotalus adamanteus case 2 ( a ), Echis coloratus ( b ), Macrovipera schweizeri ( c ). MW—protein molecular weight marker (Thermo Scientific TM , Spectra TM Multicolor Broad Range Protein Ladder, Life Technologies Czech Republic Ltd., Prague, Czech Republic). Molecular masses (kDa) are indicated on the left side. FBG—human fibrinogen standard. The sample number is provided in the top line and hours since bite in the bottom line. Red arrows mark fibrinogen fragments induced by snakebite. The chains of human fibrinogen standards are highlighted with red square boxes.

    Article Snippet: The FBG immunodetection was performed using rabbit anti-human fibrinogen polyclonal antibody (DAKO, HPST, Prague, Czech Republic; dilution 1:50,000 in 0.1% Tween/Tris-buffered saline) for 1 h at room temperature.

    Techniques: Western Blot, SDS Page, Molecular Weight, Marker